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ORIGINAL ARTICLE
Year : 2017  |  Volume : 9  |  Issue : 2  |  Page : 98-104

Antibacterial activity of four root canal sealers against Enterococcus Faecalis at 1st, 3rd, 5th, and 7th day duration: A In vitro study


1 Department of Conservative Dentistry and Endodontics, BJS Dental College and Hospital, Ludhiana, Punjab, India
2 Department of Conservative Dentistry and Endodontics, Maratha Mandal Dental College and Research Centre, Belgaum, Karnataka, India
3 Department of Microbiology, Jawaharlal Nehru Medical College, Belgaum, Karnataka, India
4 Department of Periodontology and Oral Implantology, MMCDSR, Mullana, Ambala, Haryana, India
5 Department of Oral and Maxillofacial Surgery, BJS Dental College and Hospital, Ludhiana, Punjab, India
6 Department of Prosthodontics, SKSS Dental College, Sarabha, Ludhiana, Punjab, India

Correspondence Address:
Bhuvanesh Tandon
Department of Conservative Dentistry and Endodontics, BJS Dental College and Hospital, Ludhiana, Punjab
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/IJDS.IJDS_22_17

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Background: Root filling plays an important role in control of reinfection by entombing the residual organisms through antimicrobial activity of endodontic sealers. Several endodontic sealers have also been found to have antibacterial properties, depending on their chemical contents. Aim: Aim of the present study was to assess the antibacterial activity of 4 endodontic sealers i.e AH Plus (Group 1), Sealapex (Group 2), Roekoseal (Group 3) and ZOE (Group 4) against E. faecalis at 1hr, 1st, 3rd, 5th and 7th day duration. Materials and Methods: Antimicrobial activities of four root canal sealers were evaluated against E. faecalis at various time intervals by direct contact test. Freshly mixed sealers were placed in 96-well microtitre plates. Plates were placed for incubation at 37°C and thereafter placed in microplate reader wherein optical density in each well was measured at 630 nm. Readings were taken at regular intervals. i.e after 1 hr (fresh specimen), then on 1st, 3rd, 5th and 7th day. Statistical Analysis: Data was collected by recording optical density, considering the fact that as, bacterial population increases, absorbance reading, i.e. Optical Density (O.D.) given by spectrophotometer increases. Data were recorded, then plotted and statistically analyzed using 2 way -ANOVA (Analysis of Variance) and Newman-Keuls Post hoc procedure. Result: Intragroup comparison in various groups showed greatest antibacterial effect at 1 hr, followed by a decrease in the effectiveness at 1st day, 3rd day, 5thday, 7th day. In intergroup comparison, GROUP 2 (Sealapex) showed least inhibition of bacterial growth at 1 hr interval followed by further decrease at different studied intervals. Conclusion: It was concluded that Antibacterial activity of tested endodontic sealers on E. faecalis in an ascending order was as follows: Sealapex, Roekoseal, AH Plus and Zinc Oxide Eugenol sealer.


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